Phosphomotif antibodies
Webphosphomotif antibodies, indicating the pos-sibility of direct ERK substrates. From this screen, 38 candidate targets were identi-fied by mass spectrometry (MS), includ-ing 13 new substrates that were verified by in vitro ERK phosphorylation. Further stud-ies of one of them, the nucleoporin Nup50, revealed an unexpected role of ERK signal- Webnonphosphorylatable Ser172Ala mutation abolished interaction with the antibody to the 14-3-3 binding motif, whereas a nonphosphorylatable Ser155Ala mutation abolished the interaction with the antibody to the MPK substrate motif, and mutation of either site alone did not affect the AICAR-induced phosphorylation of the other site (Fig. 3C).
Phosphomotif antibodies
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WebJan 9, 2014 · After 2D-DIGE analysis, changed protein spots were characterized for reactivity to ERK phosphomotif antibodies and identified by MS. Various types of phosphomotif … WebDec 8, 2015 · Together, using a TNIK inhibitor and phosphomotif antibody, we identify endogenous substrates of TNIK in neurons, define consensus sequences for TNIK, and suggest signaling pathways by which...
WebJan 1, 2005 · The antibody reactive with the phosphomotif generated by Akt, referred to here as the PAS (phospho Akt substrate) antibody, was purchased from Cell Signaling Technology, Beverly, MA (catalog number 9611). Rabbit antisera against peptides from the insulin-elicited phosphoproteins were generated for us by BioSource International, … Webby a recent study in which phospho-motif specific antibodies were used to enrich for peptides containing pS/pT-Q, with the goal of identifying substrates of SQ/TQ-directed kinases (e.g., ... including cell line and phosphospecific-versus-phosphomotif antibody differences. The phosphorylation sites identified in both data sets are of
WebProtocols. As the epigenetics experts, Active Motif continues to develop new methods and helpful techniques to support your research. Our goal is to ensure your success with everything from gold standard assays to emerging approaches for epigenetics analysis. Check back often as we add new protocols, technotes and guides to this resource. WebJan 4, 2013 · These antibodies are commonly used in immunoprecipitation and/or immunoblotting protocols to determine kinase-induced substrate phosphorylation. This …
WebPhosphoproteomic screen for PDK4 substrates, followed by nonphosphorylatable and phosphomimetic mutations of the PDK4 site revealed cytoplasmic GTPase, Septin 2 (SEPT2), as the key effector …
WebFeb 1, 2016 · Together, using a TNIK inhibitor and phosphomotif antibody, we identify endogenous substrates of TNIK in neurons, define consensus sequences for TNIK, and … grand turk island hurricane damageWebSep 20, 2009 · Using a phosphoproteomic approach combining the steroid receptor fusion system, IMAC, 2D-DIGE and phosphomotif-specific antibodies, we detected 38 proteins … chinese sign for born in january 18 1959WebPhosphoSitePlus Protein or Substrate Search Alternative Search Options Protein, Sequence, or Reference Search Protein, Sequence, or Reference Search: Protein Searches retrieve lists of proteins and their modification types based on protein name or ID, protein type, domain, cellular component, MW, and pI range. chinese sign for 2021WebAntiphospholipid antibodies are antibodies directed against phosphorus-fat components of your cell membranes called phospholipids, certain blood proteins that bind with … grand turk island in the bahamasWebThis study describes a method for the identification of the substrates of specific serine kinases. An antibody specific for the phosphomotif generated by the kinase is used to … chinese sign for familyWebMay 13, 2015 · The ability of anti-mHttexon1 antibodies and antibody fragments to reduce the aggregation and associated neurotoxicity has proven to be strongly dependent on the targeted epitope . Specifically targeting the Htt sequence between 1 and 17, which has been shown to play a key role in aggregation and HD pathology, seems to be an obvious choice. chinese sign for long lifechinese sign for 2023