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WebJan 13, 2010 · Step Four: Extract the DNA When you’ve extracted the liquid from the gunk, carefully pour at least 5ml (1 tsp) of the fluid into a narrow vessel, such as a clean shot glass, clear plastic vial, or test tube. (If you’re using a vessel larger than a test tube, you’ll need more fluid. Use enough to fill the container at least one-quarter full.) WebAcMNPV sequences flanking the gene in the transfer plasmid's MCS allow recombination with the flashBACTM DNA to insert the expression cassette into the polyhedron (polh) … WebAcMNPV sequences flanking the gene in the transfer plasmid's MCS allow recombination with the FlashBACTM DNA to insert the expression cassette into the polyhedron (polh) locus under the control of the polh promoter. Additionally, pOET-1NTM has been designed for the high-level expression of foreign genes fused to an N-terminal 6xHis tag, easing ... trusted protein powder brands