Earcht-eyfp

Author: pmhe

August undefined, 2024

Webinjecting AAV8-CaMKII:eArchT-EYFP into vHPC and then optogenetically inhibiting axonal . 17 . terminals of the vCA1 neurons in the respective target areas while the mice went through SDT . 18 (Fig. 1, L to N, Q to S, and fig. S4). vHPC-NAc projections, but not vHPC-OB or vHPC-BLA, 19 . were essential for social discrimination behavior. 20 WebSep 9, 2024 · For this purpose, we bilaterally injected Cre-dependent AAVrh8-hSyn1-DIO-eArchT-eYFP into MECIII-specific Cre mice (pOxr1-Cre) and unilaterally implanted a …

Ventral CA1 neurons store social memory - PMC

WebSep 30, 2016 · To investigate the potential role of vHPC and dHPC in social memory, we targeted bilateral injections of adeno-associated virus 8 (AAV8)–calcium/calmodulin … WebWe bilaterally injected either adeno-associated virus 2/5 (AAV 2/ 5 )-CaMKIIa:eArchT-eYFP or AAV 2/5 -CaMKIIa:eYFP as a control in ACC, implanted optical fibers targeting BLA, and then inhibited ... cindy mcintyre photos

Lateral Habenula Serves as a Potential Therapeutic Target for ...

WebIf you are using these viruses for the first time, please obtain a Material Transfer Agreement (MTA) by contacting Dr. Karl Deisseroth [email protected] and Charu Ramakrishnan [email protected]. Please submit a copy of your MTA along with your virus order. WebpLenti-CaMKIIa-eArchT 3.0-EYFP pAAV-CaMKIIa-eArch 3.0-EYFP pAAV-Ef1a-DIO ChETA-EYFP pAAV-Syn-ChrimsonR-tdT Map uploaded by the depositor. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. ... WebApr 4, 2024 · Expression of eArchT-eYFP was abundant in MEC-Va terminals located in the PFC, cACC and RSC (Fig. 1B, I, and fig. S2D). These mice were then subjected to contextual fear conditioning (CFC) while we delivered green light bilaterally to the different cortical areas receiving MEC-Va projections during either the conditioning period (Day-1) … cindersnap forest

Lateral habenula glutamatergic neurons projecting to the …

SOCIAL MEMORY Ventral CA1 neurons store social …

WebeArchT-EYFP into vCA1 (Fig. 2, A and B, and fig. S6). We confirmed that the vCA1 neurons speci-fically project to the NAc shell but not to the NAc core, as identified by tyrosine … Web(A to L) Coronal sections of Trpc4-Cre mice injected with AAV9-hsyn:DIO-eArchT- EYFP (A, C), AAVrh8-syn:DIO-EGFP (D to I), and AAV5-hsyn:DIO-GCaMP6f (J to L) into vCA1. cincinnati style chili historyWebSep 9, 2024 · (A) Sagittal sections of MEC and CA1 area. MECIII-Cre mice were bilaterally infected with the Cre specific AAVrh8-hSyn1-DIO-eArchT-eYFP virus and silicone linear probes with an optical fiber were implanted in both areas. (B) Examples of light stimulation during quiet awake when animals were in an open arena. Top panel: Light OFF condition. cindy phelps meller

"Web6 1. Click on a message to display the details. Alternatively, click the view all messages link to display the My messages page. 2. If required, click on the Subject or Received … " - Earcht-eyfp

Earcht-eyfp

MEMORY RESEARCH Engrams and circuits crucial for systems …

WebMay 8, 2014 · Control Data for Optogenetics, eArchT Activation at CA1 Axon Terminal Does Not Affect MEC Unit Firing, Related to Figure 6 (A) An example of MUA in dCA1 with 473 nm light illumination in an anesthetized pOxr1-Cre/eArchT-eYFP mouse. Left, blue bar indicates a period of light illumination (20 Hz for 200 ms). WebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Did you know?

Web8-CaMKII:eArchT-eYFP injection into the MEC (left) and coronal sections of PFC with MEC-Va axons expressing eYFP (green) (right). rACC, rostral ACC; PL, prelimbic cortex. (C and D) Sagittal section of MEC with CTB-labeled cells (red), immunostained with anti-PCP4 (green) and anti-NeuN (blue). PCP4 is a WebNov 18, 2024 · Third, a subset of mice injected with AAV1-DIO-ChR2-EYFP (n = 3) or AAV2/9-DIO-eArchT-EGFP (n = 4) in the OT for optogenetic behavioral experiments was additionally used for anterograde tracing ...

WebJun 4, 2024 · The role of hyperactivity of LHb neurons after PSL was then investigated by selectively inhibiting LHb Glu using an optogenetic approach (Fig. 2A). pAAV-CaMKIIα-eArchT-eYFP was infused into the right LHb and the precise and functional transduction was confirmed by the co-localization of eYFP and CaMKIIα, a marker for glutamatergic … WebThe AAVrh8-hSyn1-DIO-eArchT-eYFP plasmid was constructed by inserting the eArchT-eYFP gene fragment, which was obtained from a template, pLenti-CaMKIIa-eArchT_3.0eYFP (courtesy of Dr. Karl Deisseroth at Stanford University)

WebMar 21, 2024 · To this end, we made AAV-TetO-eArchT-eYFP-WPRE (TetO::eArchT) , expressing an inhibitory optogenetic actuator activated by green light. Four weeks after injection of TetO::eArchT in Tph2-tTA mice, we tested the effect of photoinhibition of the DRN in the CPP test (Figure 4 A–D). To assess aversive properties, we associated … WebJul 21, 2024 · d Expression of eArchT-EYFP in the DRN and median raphe nucleus. Optic fiber was placed into the DRN (scale bar 500 μm). The timeline of the experiment was exactly the same as in Fig. ...

WebJun 4, 2024 · The role of hyperactivity of LHb neurons after PSL was then investigated by selectively inhibiting LHb Glu using an optogenetic approach (Fig. 2A). pAAV-CaMKIIα …

WebApr 4, 2024 · Specifically, context A-A (trained context) vs. context A-B (neutral context) for natural recall ratios, ChR2-eYFP vs. eYFP for engram cell activation ratios, and eArchT … cindi williams hcmWebEYFP (C terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site PacI (not destroyed) 3′ cloning site BsrGI (not destroyed) 5′ sequencing … cindy schlabachWebA Place Where Members are Cherished Like Family. Discover a welcoming atmosphere and warm community to go along with our exquisite dining, premiere racquet facilities, pool … cindy o\u0027hare dog showsWebMay 8, 2014 · Control Data for Optogenetics, eArchT Activation at CA1 Axon Terminal Does Not Affect MEC Unit Firing, Related to Figure 6 (A) An example of MUA in dCA1 with 473 … cindy witherspoonWebTo this end, we made AAV-TetO-eArchT-eYFP-WPRE (TetO::eArchT) , expressing an inhibitory optogenetic actuator activated by green light. Four weeks after injection of TetO::eArchT in Tph2-tTA mice, we tested the effect of photoinhibition of the DRN in the CPP test (Figure 4A–D). To assess aversive properties, we associated green light ... cindy swift facebookWebApr 4, 2024 · Specifically, context A-A (trained context) vs. context A-B (neutral context) for natural recall ratios, ChR2-eYFP vs. eYFP for engram cell activation ratios, and eArchT-mCherry vs. mCherry for ... cindy renardWebMar 18, 2024 · The amygdala is a key structure for fear memory (Davis, 1992, Duvarci and Pare, 2014, Ehrlich et al., 2009, Maren and Fanselow, 1996) and is also involved in fear extinction memory ( Amano et al., 2010, Grewe et al., 2024, Herry et al., 2008 ). However, it is unknown whether the amygdala is the storage site of fear extinction memory and, if so ... cindy secret story age