Cytosolic translocation
WebDec 9, 2024 · Here, we demonstrate that late endosomes, a type of prelysosomal organelles, facilitate actin-cytoskeleton remodeling via cytosolic translocation of … WebMar 3, 2012 · Translocation of Parkin to damaged mitochondria induces mitophagy in many non-neuronal cell types [9–16]. However, evidence showing Parkin translocation in primary neurons is controversial [ 9 , 15 , 17 , 18 ], leaving unanswered questions as to how and where Parkin-mediated mitophagy occurs in neurons.
Cytosolic translocation
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WebMar 31, 2003 · Because C-domain translocation across the endosomal vesicle membrane requires the addition of cytosolic components to the reaction mixture, we used … WebSep 15, 2024 · Extracellular signal-regulated kinase (ERK) signaling plays a crucial role in regulating immune cell function and has been implicated in autoimmune disorders. To date, all commercially available inhibitors of ERK target upstream components, such as mitogen-activated protein (MAP) kinase/ERK kinase (MEKs), but not ERK itself. Here, we directly …
WebMar 13, 2024 · Mycobacterial cytosolic translocation was monitored by using CCF4-AM as a FRET reporter. The Blue/Green ratio was measured by comparing emissions at 450nm … WebMay 3, 2024 · YIPF5 (a mammalian ortholog of yeast Yip1p) is responsible for cytosolic DNA-induced translocation of STING from the ER and the induction of IFNβ . The transmembrane emp24 domain (TMED) family are single-spanning transmembrane proteins and have emerged as critical regulators of the early (ER-to-Golgi) and late (Golgi-to …
WebJan 8, 2024 · As another example of curvature control for the cytosolic CPP translocation, Futaki et al. reported the incorporation of a hydrophobic moiety which interacts with the lipid membrane to induce ... WebNov 4, 2024 · In this context, bacterial two-component systems, termed AB toxins, use various protein-based membrane translocation mechanisms to deliver toxins into cells, and these mechanisms could provide new insights into the development of bio-based drug delivery systems. ... which is part of the cytosolic membrane fusion complex, termed …
WebDec 1, 2024 · Here, we demonstrate that late endosomes, a type of prelysosomal organelles, facilitate actin-cytoskeleton remodeling via cytosolic translocation of immature protease cathepsin D (cathD) during...
WebApr 13, 2024 · Cytosolic access of SCPNs evaluated with GIGT assay (A) Schematic illustration of the workflow of GIGT assay. (B) Representative CLSM images of GFP nuclear translocation by incubating DEX-tagged compact (blue frame) or sparse (red frame) SCPNs with HeLa, HEK293, B16-F10 and 3T3-L1. how many weeks are in 16 yearsWebApr 24, 2024 · Mycobacterial cytosolic translocation was measured by CCF-4 FRET assay as previously described (17, 22, 50). Briefly, RAW264.7 cells were plated in triplicate in a … how many weeks are in 15 monthsWebMay 1, 2004 · Increased cytosolic Ca2+ and calcineurin activation stimulated Bad translocation since BAPTA [bis-(o-aminophenoxy)ethane-N, N, N', N'-tetra-acetic acid], an intracellular Ca2+ chelator, and cyclosporin A, a calcineurin inhibitor, significantly reduced translocation. BAPTA also blocked release of cytochrome c from mitochondria as well … how many weeks are in 17 yearsWebJan 21, 2024 · Inactivation of Cdc48 leads to a formation of stalled retro-translocation complex containing Hrd1, Usa1, Hrd3, Der1, the 26S proteasome, Yos9, ubiquitinated substrates, and Cdc48. ... Cytosolic chaperones, such as the Hsp70 Ssa1 and the cytosolic Hsp40s Ydj1 and Hlj1, facilitate substrate recognition [53,54,55]. The degrons … how many weeks are in 19 daysWebJul 16, 2024 · The availability of intracellular, stabilized β-catenin, a transcription factor coactivator, is tightly regulated; β-catenin is translocated into the nucleus in response to Wnt ligand binding to its cell membrane receptors. Here we show that Wnt signal activation in mammalian cells activates intracellular mobilization of connexin 43 (Cx43), which … how many weeks are in 17 monthsWebDec 9, 2024 · Considering that enhancing potential cytosolic translocation evoked lamellipodia-distribution of cathD and increased cofilin activity (Fig. 4, G and H), we … how many weeks are in 1 yrWebMar 13, 2024 · In the present study, using liposome-leakage and cytotoxicity assays, LC-MS/MS–based proteomics, and CCF-4 FRET analysis, we obtained evidence that the N α -acetylation of the Thr-2 residue on... how many weeks are in 18 years